|
KMID : 1161519990030020207
|
|
Animal Cells and Systems
1999 Volume.3 No. 2 p.207 ~ p.213
|
|
|
Effects of retinoic acid and cAMP on the differentiation of Naegleria gruberi amoebas into flagellates
|
|
Kim Bo-Ra
Kim Hong-Kyoung
Kim Dae-Myoung
Chung In-Kwon
Kim Young-Min
Cho Jin-Won
Lee Joo-Hun
|
|
|
Abstract
|
|
|
|
During the differentiation of Naegleria gruberi amoebas into flagellates, the amoebas undergo sequential changes in cell shape and form new cellular organelles. To understand the nature of the signal which initiates this differentiation and the signal transduction pathway, we treated cells with four agents, PMA, retinoic acid (RA), okadaic acid, and cAMP. Retinoic acid and cAMP had specific effects on the differentiation of N. gruberi depending on the time of the drug treatment. Addition of (100 μM) retinoic acid at the initiation of differentiation inhibited differentiation by blocking the transcription of differentiation specific genes (e.g., (β?tubulin). This inhibition of differentiation by retinoic acid was overcome by co?treatment with cAMP (or dbcAMP, 20 μM). Addition of retinoic acid at later stages (30 and 70 min) had no effect on the transcriptional regulation of the β?tubulin gene, however the differentiation was inhibited by different degrees. Co?treatment of cAMP at these stages did not overcome the inhibitory effect of retinoic acid. These results suggest that the role of retinoic acid as a transcriptional regulator might be conserved throughout the evolution of eukaryotes
|
|
|
KEYWORD
|
|
|
Naegleria gruberi differentiation, Retinoic acid, cAMP, Transcription
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|